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A DNA-Assisted Binding Assay for Weak Protein-Protein Interactions
We describe a new method for quantitating weak interactions between proteins in which the weak interaction is “assisted” by a known DNA:DNA interaction. Oligonucleotides, which are conjugated to the proteins of interest, contain short complementary DNA sequences that provide additional binding energ...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2790015/ https://ncbi.nlm.nih.gov/pubmed/19815018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.09.064 |
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