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Increasing Charge While Preserving Noncovalent Protein Complexes for ESI-MS
Increased multiple charging of native proteins and noncovalent protein complexes is observed in electrospray ionization (ESI) mass spectra obtained from nondenaturing protein solutions containing up to 1% (v/v) m-nitrobenzyl alcohol (m-NBA). The increases in charge ranged from 8% for the 690 kDa α(7...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2789282/ https://ncbi.nlm.nih.gov/pubmed/19101165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2008.11.013 |
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