RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation

BACKGROUND: Polymerase chain reaction (PCR) is very useful in many areas of molecular biology research. It is commonly observed that PCR success is critically dependent on design of an effective primer pair. Current tools for primer design do not adequately address the problem of PCR failure due to...

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Main Authors: Piriyapongsa, Jittima, Ngamphiw, Chumpol, Assawamakin, Anunchai, Wangkumhang, Pongsakorn, Suwannasri, Payiarat, Ruangrit, Uttapong, Agavatpanitch, Gallissara, Tongsima, Sissades
Format: Artigo
Language:Inglês
Published: BioMed Central 2009
Subjects:
Proceedings
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2788391/
https://ncbi.nlm.nih.gov/pubmed/19958502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-10-S3-S4
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