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DLA-Based Strategies for Cloning Insertion Mutants: Cloning the gl4 Locus of Maize Using Mu Transposon Tagged Alleles

Digestion–ligation–amplification (DLA), a novel adaptor-mediated PCR-based method that uses a single-stranded oligo as the adaptor, was developed to overcome difficulties of amplifying unknown sequences flanking known DNA sequences in large genomes. DLA specifically overcomes the problems associated...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Liu, Sanzhen, Dietrich, Charles R., Schnable, Patrick S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Genetics Society of America 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2787415/
https://ncbi.nlm.nih.gov/pubmed/19805815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.109.108936
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