Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipid

Cell-free expression has become a highly promising tool for the efficient production of membrane proteins. In this study, we used a dialysis-based Escherichia coli cell-free system for the production of a membrane protein actively integrated into liposomes. The membrane protein was the light-driven...

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Autors principals: Shimono, Kazumi, Goto, Mie, Kikukawa, Takashi, Miyauchi, Seiji, Shirouzu, Mikako, Kamo, Naoki, Yokoyama, Shigeyuki
Format: Artigo
Idioma:Inglês
Publicat: Wiley Subscription Services, Inc., A Wiley Company 2009
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2786979/
https://ncbi.nlm.nih.gov/pubmed/19746358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.230
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