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A HUPO test sample study reveals common problems in mass spectrometry-based proteomics

We carried out a test sample study to try to identify errors leading to irreproducibility, including incompleteness of peptide sampling, in LC-MS-based proteomics. We distributed a test sample consisting of an equimolar mix of 20 highly purified recombinant human proteins, to 27 laboratories for ide...

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Detalhes bibliográficos
Main Authors: Bell, Alexander W., Deutsch, Eric W., Au, Catherine E., Kearney, Robert E., Beavis, Ron, Sechi, Salvatore, Nilsson, Tommy, Bergeron, John J.M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2785450/
https://ncbi.nlm.nih.gov/pubmed/19448641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1333
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