SCHEMA Recombination of a Fungal Cellulase Uncovers a Single Mutation That Contributes Markedly to Stability

A quantitative linear model accurately (R(2) = 0.88) describes the thermostabilities of 54 characterized members of a family of fungal cellobiohydrolase class II (CBH II) cellulase chimeras made by SCHEMA recombination of three fungal enzymes, demonstrating that the contributions of SCHEMA sequence...

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Detalhes bibliográficos
Main Authors: Heinzelman, Pete, Snow, Christopher D., Smith, Matthew A., Yu, Xinlin, Kannan, Arvind, Boulware, Kevin, Villalobos, Alan, Govindarajan, Sridhar, Minshull, Jeremy, Arnold, Frances H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Accelerated Publication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2785310/
https://ncbi.nlm.nih.gov/pubmed/19625252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.C109.034058
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