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Purification and Mechanism of Human Aldehyde Oxidase Expressed in Escherichia coli
Human aldehyde oxidase 1 (AOX1) has been subcloned into a vector suitable for expression in Escherichia coli, and the protein has been expressed. The resulting protein is active, with sulfur being incorporated in the molybdopterin cofactor. Expression levels are modest, but 1 liter of cells supplies...
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Main Authors: | , , , , , |
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Format: | Artigo |
Jezik: | Inglês |
Izdano: |
The American Society for Pharmacology and Experimental Therapeutics
2009
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Teme: | |
Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2784701/ https://ncbi.nlm.nih.gov/pubmed/19741035 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.109.029520 |
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