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Purification and Mechanism of Human Aldehyde Oxidase Expressed in Escherichia coli

Human aldehyde oxidase 1 (AOX1) has been subcloned into a vector suitable for expression in Escherichia coli, and the protein has been expressed. The resulting protein is active, with sulfur being incorporated in the molybdopterin cofactor. Expression levels are modest, but 1 liter of cells supplies...

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Bibliografske podrobnosti
Main Authors: Alfaro, Joshua F., Joswig-Jones, Carolyn A., Ouyang, Wenyun, Nichols, Joseph, Crouch, Gregory J., Jones, Jeffrey P.
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Pharmacology and Experimental Therapeutics 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2784701/
https://ncbi.nlm.nih.gov/pubmed/19741035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.109.029520
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