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Purification and Mechanism of Human Aldehyde Oxidase Expressed in Escherichia coli

Human aldehyde oxidase 1 (AOX1) has been subcloned into a vector suitable for expression in Escherichia coli, and the protein has been expressed. The resulting protein is active, with sulfur being incorporated in the molybdopterin cofactor. Expression levels are modest, but 1 liter of cells supplies...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Alfaro, Joshua F., Joswig-Jones, Carolyn A., Ouyang, Wenyun, Nichols, Joseph, Crouch, Gregory J., Jones, Jeffrey P.
פורמט: Artigo
שפה:Inglês
יצא לאור: The American Society for Pharmacology and Experimental Therapeutics 2009
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2784701/
https://ncbi.nlm.nih.gov/pubmed/19741035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.109.029520
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