Combining Single-Molecule Optical Trapping and Small-Angle X-Ray Scattering Measurements to Compute the Persistence Length of a Protein ER/K α-Helix

A relatively unknown protein structure motif forms stable isolated single α-helices, termed ER/K α-helices, in a wide variety of proteins and has been shown to be essential for the function of some molecular motors. The flexibility of the ER/K α-helix determines whether it behaves as a force transdu...

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Main Authors: Sivaramakrishnan, S., Sung, J., Ali, M., Doniach, S., Flyvbjerg, H., Spudich, J.A.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Protein
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2784555/
https://ncbi.nlm.nih.gov/pubmed/19948129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.09.009
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