Expression and purification of recombinant human coagulation factor VII fused to a histidine tag using Gateway technology

BACKGROUND: Factor VII (FVII) is a plasma glycoprotein that participates in the coagulation process leading to the generation of fibrin. The aim of this study was to construct, express and purify recombinant FVII fused to a polyhistidine (his) tag using Gateway technology. METHODS: To construct the...

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Bibliografiske detaljer
Main Authors: Halabian, Raheleh, Fathabad, Mahdi Edalati, Masroori, Nasser, Roushandeh, Amaneh Mohammadi, Saki, Sasan, Amirizadeh, Nasser, Najafabadi, Ali Jahanian, Gharehbaghian, Ahmad, Roudkenar, Mehryar Habibi
Format: Artigo
Sprog:Inglês
Udgivet: Edizioni SIMTI SIMTI Servizi Srl 2009
Fag:
Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2782808/
https://ncbi.nlm.nih.gov/pubmed/20011642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2450/2009.0081-08
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