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Rescue and characterization of episomally replicating DNA from the moss Physcomitrella

The moss Physcomitrella is unique among plants in that it permits efficient gene targeting by homologous recombination. Furthermore, transformed DNA can replicate episomally in Physcomitrella. Here we show that episomally replicating DNA can be rescued back into Escherichia coli, and we use such res...

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Bibliographic Details
Main Authors: Murén, Eva, Nilsson, Anders, Ulfstedt, Mikael, Johansson, Monika, Ronne, Hans
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2009
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2780769/
https://ncbi.nlm.nih.gov/pubmed/19892729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0908037106
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