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Rescue and characterization of episomally replicating DNA from the moss Physcomitrella

The moss Physcomitrella is unique among plants in that it permits efficient gene targeting by homologous recombination. Furthermore, transformed DNA can replicate episomally in Physcomitrella. Here we show that episomally replicating DNA can be rescued back into Escherichia coli, and we use such res...

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Autori principali: Murén, Eva, Nilsson, Anders, Ulfstedt, Mikael, Johansson, Monika, Ronne, Hans
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2780769/
https://ncbi.nlm.nih.gov/pubmed/19892729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0908037106
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