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Red-Mediated Transposition and Final Release of the Mini-F Vector of a Cloned Infectious Herpesvirus Genome

Bacterial artificial chromosomes (BACs) are well-established cloning vehicles for functional genomics and for constructing targeting vectors and infectious viral DNA clones. Red-recombination-based mutagenesis techniques have enabled the manipulation of BACs in Escherichia coli without any remaining...

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Detalhes bibliográficos
Main Authors: Wussow, Felix, Fickenscher, Helmut, Tischer, B. Karsten
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2780728/
https://ncbi.nlm.nih.gov/pubmed/19997639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0008178
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