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Determination of gene expression patterns using high-throughput RNA in situ hybridization to whole-mount Drosophila embryos
We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into anti-sense RNA probes by in vitro transcription. The quality of ea...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2780369/ https://ncbi.nlm.nih.gov/pubmed/19360017 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.55 |
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