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Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae
BACKGROUND: Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2776018/ https://ncbi.nlm.nih.gov/pubmed/19874630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-10-99 |
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