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Preparation of Biologically Active Subcellular Fractions Using the Balch Homogenizer
Obtaining vesicular fractions from cell lines or animal tissue is both time and technically intensive. The presence of plasma membrane and nuclear contaminants within a preparation is often dependent upon the method of homogenization and is usually mitigated through the use of density gradients. We...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2774742/ https://ncbi.nlm.nih.gov/pubmed/19622341 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.024 |
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