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TILLING in Lotus japonicus Identified Large Allelic Series for Symbiosis Genes and Revealed a Bias in Functionally Defective Ethyl Methanesulfonate Alleles toward Glycine Replacements

We have established tools for forward and reverse genetic analysis of the legume Lotus (Lotus japonicus). A structured population of M2 progeny of 4,904 ethyl methanesulfonate-mutagenized M1 embryos is available for single nucleotide polymorphism mutation detection, using a TILLING (for Targeting In...

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Detalhes bibliográficos
Main Authors: Perry, Jillian, Brachmann, Andreas, Welham, Tracey, Binder, Andreas, Charpentier, Myriam, Groth, Martin, Haage, Kristina, Markmann, Katharina, Wang, Trevor L., Parniske, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2773058/
https://ncbi.nlm.nih.gov/pubmed/19641028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.109.142190
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