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Development and Use of a Selectable, Broad-Host-Range Reporter Transposon for Identifying Environmentally Regulated Promoters in Bacteria
This report describes the development and use of TnKnXSp, a selectable broad-host-range reporter transposon with a promoterless aphA gene. Insertion of TnKnXSp into the chromosome of a kanamycin-susceptible bacterium confers resistance to kanamycin only if aphA is transcribed from an active promoter...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2772819/ https://ncbi.nlm.nih.gov/pubmed/19146571 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1574-6968.2008.01430.x |
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