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Two Novel Techniques for Determination of Polysaccharide Cross-Links Show that Crh1p and Crh2p Attach Chitin to both β(1-6)- and β(1-3)Glucan in the Saccharomyces cerevisiae Cell Wall
Previous work, using solubilization of yeast cell walls by carboxymethylation, before or after digestion with β(1-3)- or β(1-6)glucanase, followed by size chromatography, showed that the transglycosylases Crh1p and Crh2p/Utr2p were redundantly required for the attachment of chitin to β(1-6)glucan. W...
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
2009
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2772413/ https://ncbi.nlm.nih.gov/pubmed/19734368 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00228-09 |
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