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A yeast two-hybrid smart-pool-array system for protein-interaction mapping

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae...

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Detalhes bibliográficos
Main Authors: Jin, Fulai, Avramova, Larisa, Huang, Jing, Hazbun, Tony
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771206/
https://ncbi.nlm.nih.gov/pubmed/17450148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth1042
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