Crystallization and preliminary X-ray analysis of ZHE1, a hatching enzyme from the zebrafish Danio rerio

The hatching enzyme of the zebrafish, ZHE1 (29.3 kDa), is a zinc metallo­protease that catalyzes digestion of the egg envelope (chorion). ZHE1 was heterologously expressed in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as the precipitant. Tw...

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Detalhes bibliográficos
Main Authors: Okada, Akitoshi, Nagata, Koji, Sano, Kaori, Yasumasu, Shigeki, Kubota, Keiko, Ohtsuka, Jun, Iuchi, Ichiro, Tanokura, Masaru
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765890/
https://ncbi.nlm.nih.gov/pubmed/19851011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109033016
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