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Expression, purification, crystallization and preliminary X-ray analysis of para-nitrophenol 4-­monooxygenase from Pseudomonas putida DLL-­E4

Para-nitrophenol 4-monooxygenase (PnpA) plays an important role in bacterial degradation of para-nitrophenol by oxidative release of the nitro group from the aromatic ring to form p-benzoquinone. In order to understand the structural basis of the function of this enzyme, PnpA was cloned, expressed i...

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Detalhes bibliográficos
Main Authors: Liu, Weidong, Shen, Wenjing, Zhao, Xiaoli, Cao, Hui, Cui, Zhongli
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765886/
https://ncbi.nlm.nih.gov/pubmed/19851007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109032370
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