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Cloning of Salmonella enterica Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in Escherichia coli Confers the Ability To Attach to Eukaryotic Cell Lines

The gene for the Salmonella enterica serovar Enteritidis fimbrial protein SefA was cloned into an Escherichia coli surface expression vector and confirmed by Western blot assay. E. coli clones expressing SefA attached to avian ovary granulosa cells and HEp-2 cells, providing evidence for the involve...

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Detalhes bibliográficos
Main Authors: Rank, Douglas L., Saeed, Mahdi A., Muriana, Peter M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765155/
https://ncbi.nlm.nih.gov/pubmed/19700549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00639-09
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