Inducible-Expression Plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans

We have developed a stable isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable r...

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Autori principali: Ind, Alice C., Porter, Steven L., Brown, Mostyn T., Byles, Elaine D., de Beyer, Jennifer A., Godfrey, Scott A., Armitage, Judith P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2009
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765152/
https://ncbi.nlm.nih.gov/pubmed/19684165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01587-09
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