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Discrimination between docking and fusion of liposomes reconstituted with neuronal SNARE-proteins using FCS

Neuronal exocytosis is mediated by the SNARE proteins synaptobrevin 2/VAMP, syntaxin 1A, and SNAP-25A. While it is well-established that these proteins mediate membrane fusion after reconstitution in artificial membranes, it has so far been difficult to monitor intermediate stages of the reaction. U...

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Main Authors: Cypionka, Anna, Stein, Alexander, Hernandez, Javier Matias, Hippchen, Hendrik, Jahn, Reinhard, Walla, Peter J.
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2764736/
https://ncbi.nlm.nih.gov/pubmed/19843696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0906677106
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