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Measurement of Dissociation Rate of Biomolecular Complexes Using Capillary Electrophoresis

Fluorescence anisotropy (FA), non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) and high-speed capillary electrophoresis (CE) were evaluated for measuring dissociation kinetics of peptide-protein binding systems. Fyn-SH3-SH2, a protein construct consisting of the Src homolog...

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Detalhes bibliográficos
Main Authors: Yang, Peilin, Mao, Yingwei, Lee, Angel W.-M, Kennedy, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2763920/
https://ncbi.nlm.nih.gov/pubmed/19148904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200800397
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