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Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions

The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged s...

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Bibliographic Details
Main Authors: Prabhat, Prashant, Ram, Sripad, Ward, E. Sally, Ober, Raimund J.
Format: Artigo
Language:Inglês
Published: 2004
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2761735/
https://ncbi.nlm.nih.gov/pubmed/15631134
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