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Removal of Bound Triton X-100 from Purified Bovine Heart Cytochrome bc(1)

Cytochrome bc(1) isolated from Triton X-100 solubilized mitochondrial membranes contains up to 120 nmol of Triton X-100 bound per nmol of the enzyme. Purified cytochrome bc(1) is fully active; however, protein bound Triton X-100 significantly interferes with structural studies of the enzyme. Removal...

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Main Authors: Varhač, Rastislav, Robinson, Neal C., Musatov, Andrej
Formato: Artigo
Idioma:Inglês
Publicado: 2009
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2760635/
https://ncbi.nlm.nih.gov/pubmed/19733142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.08.042
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