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Removal of Bound Triton X-100 from Purified Bovine Heart Cytochrome bc(1)
Cytochrome bc(1) isolated from Triton X-100 solubilized mitochondrial membranes contains up to 120 nmol of Triton X-100 bound per nmol of the enzyme. Purified cytochrome bc(1) is fully active; however, protein bound Triton X-100 significantly interferes with structural studies of the enzyme. Removal...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2760635/ https://ncbi.nlm.nih.gov/pubmed/19733142 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.08.042 |
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