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Rapid Screening for Temperature-Sensitive Alleles in Plants
We developed a simple and fast method to identify temperature-sensitive alleles of essential plant genes. We used primary and tertiary structure information to identify residues in the core of the protein of interest. These residues were mutated and tested for temperature sensitivity, taking advanta...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society of Plant Biologists
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2754644/ https://ncbi.nlm.nih.gov/pubmed/19666707 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.109.143727 |
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