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Imaging Specific Cell Surface Protease Activity in Living Cells Using Reengineered Bacterial Cytotoxins

The scarcity of methods to visualize the activity of individual cell surface proteases in situ has hampered basic research and drug development efforts. In this chapter, we describe a simple, sensitive, and noninvasive assay that uses nontoxic reengineered bacterial cytotoxins with altered protease...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hobson, John P., Liu, Shihui, Leppla, Stephen H., Bugge, Thomas H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2753202/
https://ncbi.nlm.nih.gov/pubmed/19377967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-003-8_7
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