Use of Chimeric DNA-RNA Primers in Quantitative PCR for Detection of Ehrlichia canis and Babesia canis

To overcome the problem of nonspecific by-products in quantitative PCR (qPCR) assays, we constructed DNA-RNA chimeric primers and evaluated their use in the detection and quantification of the Ehrlichia canis 16S rRNA, Babesia canis Hsp70, and canine β-actin genes. Several RNA bases were incorporate...

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Detaylı Bibliyografya
Asıl Yazarlar: Peleg, Ofer, Baneth, Gad, Eyal, Osnat, Inbar, Jacob, Harrus, Shimon
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2009
Konular:
Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2753072/
https://ncbi.nlm.nih.gov/pubmed/19633128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00720-09
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