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Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung
Rapid genome-wide identification of genes required for infection would expedite studies of bacterial pathogens. We developed genome-scale “negative selection” technology that combines high-density transposon mutagenesis and massively parallel sequencing of transposon/chromosome junctions in a mutant...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2752563/ https://ncbi.nlm.nih.gov/pubmed/19805314 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0906627106 |
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