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NMR Chemical Exchange as a Probe for Ligand-Binding Kinetics in a Theophylline-Binding RNA Aptamer
The apparent on- and off-rate constants for theophylline binding to its RNA aptamer in the absence of Mg(2+) were determined here by 2D (1)H-(1)H NMR ZZ-exchange spectroscopy. Analysis of the build-up rate of the exchange cross peaks for several base-paired imino protons in the RNA yielded an appare...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2752443/ https://ncbi.nlm.nih.gov/pubmed/19317486 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja900695m |
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