Loading...

NMR Chemical Exchange as a Probe for Ligand-Binding Kinetics in a Theophylline-Binding RNA Aptamer

The apparent on- and off-rate constants for theophylline binding to its RNA aptamer in the absence of Mg(2+) were determined here by 2D (1)H-(1)H NMR ZZ-exchange spectroscopy. Analysis of the build-up rate of the exchange cross peaks for several base-paired imino protons in the RNA yielded an appare...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Main Authors: Latham, Michael P., Zimmermann, Grant R., Pardi, Arthur
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2752443/
https://ncbi.nlm.nih.gov/pubmed/19317486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja900695m
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!