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Rapid and simple ribozymic aminoacylation using 3 conserved nucleotides
Selection-amplification finds new RNA enzymes (ribozymes) among randomized RNAs with flanking unvaried sequences (primer complements). Precise removal of 3′-primer before reaction selected aminoacylation from PheAMP in 3 cycles, yielding active RNAs (k(cat) = 12-20 min(-1)) using only three conserve...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2750092/ https://ncbi.nlm.nih.gov/pubmed/19351205 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja809419f |
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