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Cloning, Expression, and Characterization of a Peculiar Choline-Binding β-Galactosidase from Streptococcus mitis
A Streptococcus mitis genomic DNA fragment carrying the SMT1224 gene encoding a putative β-galactosidase was identified, cloned, and expressed in Escherichia coli. This gene encodes a protein 2,411 amino acids long with a predicted molecular mass of 268 kDa. The deduced protein contains an N-termina...
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Hauptverfasser: | , , , , |
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Format: | Artigo |
Sprache: | Inglês |
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American Society for Microbiology (ASM)
2009
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Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2747876/ https://ncbi.nlm.nih.gov/pubmed/19633119 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00618-09 |
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