Discovery of [NiFe] Hydrogenase Genes in Metagenomic DNA: Cloning and Heterologous Expression in Thiocapsa roseopersicina

Using a metagenomics approach, we have cloned a piece of environmental DNA from the Sargasso Sea that encodes an [NiFe] hydrogenase showing 60% identity to the large subunit and 64% to the small subunit of a Thiocapsa roseopersicina O(2)-tolerant [NiFe] hydrogenase. The DNA sequence of the hydrogena...

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Bibliografiske detaljer
Main Authors: Maróti, Gergely, Tong, Yingkai, Yooseph, Shibu, Baden-Tillson, Holly, Smith, Hamilton O., Kovács, Kornél L., Frazier, Marvin, Venter, J. Craig, Xu, Qing
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Environmental Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2747870/
https://ncbi.nlm.nih.gov/pubmed/19633107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00580-09
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