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Enhanced Bacterial Protein Expression During Auto-induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
The auto-induction method of protein expression in E. coli is based on diauxic growth resulting from dynamic function of lac operon regulatory elements (lacO and LacI) in mixtures of glucose, glycerol and lactose. The results show that successful execution of auto-induction is strongly dependent on...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2747370/ https://ncbi.nlm.nih.gov/pubmed/17506520 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bp070011x |
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