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Subunit counting in membrane-bound proteins
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of in...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2007
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2744285/ https://ncbi.nlm.nih.gov/pubmed/17369835 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/NMETH1024 |
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