Isoform discovery by targeted cloning, “deep well” pooling, and parallel sequencing

Describing the “ORFeome” of an organism, including all major isoforms, is essential for a systems understanding of any species; however, conventional cloning and sequencing approaches are prohibitively costly and labor-intensive. We describe a potentially genome-wide methodology for efficiently capt...

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Detalhes bibliográficos
Main Authors: Salehi-Ashtiani, Kourosh, Yang, Xinping, Derti, Adnan, Tian, Weidong, Hao, Tong, Lin, Chenwei, Makowski, Kathryn, Shen, Lei, Murray, Ryan R, Szeto, David, Tusneem, Nadeem, Smith, Douglas R, Cusick, Michael E, Hill, David E, Roth, Frederick P, Vidal, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743938/
https://ncbi.nlm.nih.gov/pubmed/18552854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1224
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