Changes in Electrostatic Surface Potential of Na(+)/K(+)-ATPase Cytoplasmic Headpiece Induced by Cytoplasmic Ligand(s) Binding

A set of single-tryptophan mutants of the Na(+)/K(+)-ATPase isolated, large cytoplasmic loop connecting transmembrane helices M4 and M5 (C45) was prepared to monitor effects of the natural cytoplasmic ligands (i.e., Mg(2+) and/or ATP) binding. We introduced a novel method for the monitoring of the c...

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Bibliografiske detaljer
Main Authors: Kubala, Martin, Grycova, Lenka, Lansky, Zdenek, Sklenovsky, Petr, Janovska, Marika, Otyepka, Michal, Teisinger, Jan
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2009
Fag:
Protein
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2741587/
https://ncbi.nlm.nih.gov/pubmed/19751681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.002
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