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Single-cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary
Single-cell transfection of adherent cells has been accomplished using single-cell electroporation (SCEP) with a pulled capillary. HEPES-buffered physiological saline solution containing pEGFP plasmid at a low concentration (0.16 ∼ 0.78 µg/µL) filled a 15 cm long capillary with a tip opening of 2 µm...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2739742/ https://ncbi.nlm.nih.gov/pubmed/19351139 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac900265f |
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