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Improved method for trypsin digestion of Paraplast sections before immunofluorescence staining.

Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permit...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Rowse-Eagle, D, Watson, H D, Tignor, G H
Format: Artigo
Langue:Inglês
Publié: 1981
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC273931/
https://ncbi.nlm.nih.gov/pubmed/7016918
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