RNA Colony Blot Hybridization Method for Enumeration of Culturable Vibrio cholerae and Vibrio mimicus Bacteria

A species-specific RNA colony blot hybridization protocol was developed for enumeration of culturable Vibrio cholerae and Vibrio mimicus bacteria in environmental water samples. Bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobili...

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Bibliographic Details
Main Authors: Grim, Christopher J., Zo, Young-Gun, Hasan, Nur A., Ali, Afsar, Chowdhury, Wasimul B., Islam, Atiqul, Rashid, Mohammed H., Alam, Munirul, Morris, J. Glenn, Huq, Anwar, Colwell, Rita R.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737924/
https://ncbi.nlm.nih.gov/pubmed/19561182
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02007-08
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