RNA Colony Blot Hybridization Method for Enumeration of Culturable Vibrio cholerae and Vibrio mimicus Bacteria

A species-specific RNA colony blot hybridization protocol was developed for enumeration of culturable Vibrio cholerae and Vibrio mimicus bacteria in environmental water samples. Bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobili...

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Detalhes bibliográficos
Main Authors: Grim, Christopher J., Zo, Young-Gun, Hasan, Nur A., Ali, Afsar, Chowdhury, Wasimul B., Islam, Atiqul, Rashid, Mohammed H., Alam, Munirul, Morris, J. Glenn, Huq, Anwar, Colwell, Rita R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737924/
https://ncbi.nlm.nih.gov/pubmed/19561182
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02007-08
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