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Ultrabright Fluorescein-Labeled Antibodies Near Silver Metallic Surfaces

Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when l...

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Bibliographic Details
Main Authors: Lakowicz, Joseph R., Malicka, Joanna, Huang, Jun, Gryczynski, Zygmunt, Gryczynski, Ignacy
Format: Artigo
Language:Inglês
Published: 2004
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737397/
https://ncbi.nlm.nih.gov/pubmed/15274090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.20098
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