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A novel expression system for artificial miRNAs containing no endogenous miRNA precursor sequences

Recently, artificial microRNA (miRNA)-mediated RNA interference (RNAi) systems have been developed as useful tools to study gene functions. We report an artificial miRNA precursor motif (AMPM) containing several restriction sites in the loop and in the extended stem structures, and generated a vecto...

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Detalhes bibliográficos
Main Authors: Shibata, Atsushi, Iwaki, Akiko, Fukumaki, Yasuyuki
Formato: Artigo
Idioma:Inglês
Publicado em: Library Publishing Media 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737215/
https://ncbi.nlm.nih.gov/pubmed/19771221
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