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Principles of a quantitative assay for bacterial endotoxins in blood that uses Limulus lysate and a chromogenic substrate.

Some factors affecting the use of chromogenic substrates with Limulus lysate for assaying bacterial endotoxins in blood have been assessed. It was found that endogenous amidases, which degrade the substrate, could be inactivated by heating serum at 60 degrees C for 15 min. Endotoxin was found not to...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Autor Principal: Webster, C J
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1980
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC273663/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7276141/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.12.5.644-650.1980
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