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Thiol protection in membrane protein purifications: A study with phage holins
The lambda holin, or S105, is a small cytoplasmic membrane protein that controls the timing of host lysis. Using thiol-specific reagents, we determined that the single cysteine residue within S105 was heterogeneously modified during membrane extraction and subsequent immobilized metal ion chromatogr...
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| Huvudupphovsmän: | , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2009
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2735789/ https://ncbi.nlm.nih.gov/pubmed/19401187 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.04.031 |
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