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Thiol protection in membrane protein purifications: A study with phage holins

The lambda holin, or S105, is a small cytoplasmic membrane protein that controls the timing of host lysis. Using thiol-specific reagents, we determined that the single cysteine residue within S105 was heterogeneously modified during membrane extraction and subsequent immobilized metal ion chromatogr...

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Main Authors: Dewey, Jill S., Struck, Douglas K., Young, Ry
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2735789/
https://ncbi.nlm.nih.gov/pubmed/19401187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.04.031
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