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Trifluoromethanesulfonic acid-based proteomic analysis of cell wall and secreted proteins of the ascomycetous fungi Neurospora crassa and Candida albicans

Cell wall proteins from purified Candida albicans and Neurospora crassa cell walls were released using trifluoromethanesulfonic acid (TFMS) which cleaves the cell wall glucan/chitin matrix and deglycosylates the proteins. The cell wall proteins were then characterized by SDS PAGE and identified by p...

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Detalhes bibliográficos
Main Authors: Maddi, Abhiram, Bowman, Shaun M., Free, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2728151/
https://ncbi.nlm.nih.gov/pubmed/19555771
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fgb.2009.06.005
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