A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Nipah virus (NiV) and Hendra virus (HeV) are the only paramyxoviruses requiring Biosafety Level 4 (BSL-4) containment. Thus, study of henipavirus entry at less than BSL-4 conditions necessitates the use of cell-cell fusion or pseudotyped reporter virus assays. Yet, these surrogate assays may not ful...

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Detalhes bibliográficos
Main Authors: Wolf, Mike C, Wang, Yao, Freiberg, Alexander N, Aguilar, Hector C, Holbrook, Michael R, Lee, Benhur
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2727953/
https://ncbi.nlm.nih.gov/pubmed/19646266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-6-119
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