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Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97....

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Bibliographic Details
Main Authors: Cunha, Cristina W., Otto, Lisa, Taus, Naomi S., Knowles, Donald P., Li, Hong
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725674/
https://ncbi.nlm.nih.gov/pubmed/19494077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00997-09
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