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Consensus Amplification and Novel Multiplex Sequencing Method for S Segment Species Identification of 47 Viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus Genera of the Family Bunyaviridae

A reverse transcription-PCR (RT-PCR) assay was designed, according to previously determined and newly derived genetic data, to target S genomic segments of 47 viruses, including 29 arthropod-borne human pathogens, of the family Bunyaviridae. The analytical sensitivity of the presented assay was eval...

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Hlavní autoři: Lambert, Amy J., Lanciotti, Robert S.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725646/
https://ncbi.nlm.nih.gov/pubmed/19535518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00182-09
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